Human PBMC single-cell dataset (~1000 cells)

Fresh PBMCs were isolated from a healthy donor using the EasySep™ Direct Human PBMC Isolation Kit with >95% viability confirmed by trypan blue exclusion. The cell suspension was processed using the ArgenTag Single-Cell RNA Library Kit for Long-Read Sequencing.

During cDNA amplification, the PCR reaction was split into ten aliquots (40 µL each) under standard cycling conditions. One aliquot was reamplified for five additional cycles, generating a cDNA pool representative of 1/10 fraction of total cells (~1000). Library preparation employed the Oxford Nanopore LSK-114 amplicon sequencing kit following ArgenTag protocol guidelines. The library was sequenced on a PromethION 2 Solo device using a P2 flow cell.